内脂素（VF）检测试剂盒说明书

       内脂素(VF)检测试剂盒
　　适用生物 Homo sapiens (Human，人) 内脂素(VF)检测试剂盒检测范围 31.25-2000pg/mL 灵敏度 13.5pg/mL 样本类型 Serum, plasma and other biological fluids. 实验时长 4.5h 实验方法 双抗夹心法 内脂素(VF)检测试剂盒规格 96T
　　内脂素(VF)检测试剂盒 ELISA Kit for Visfatin (VF)
　　FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
　　Specificity
　　This assay has high sensitivity and excellent specificity for detection of Visfatin (VF). No significant cross-reactivity or interference between Visfatin (VF) and analogues was observed.
　　Recovery
　　Matrices listed below were spiked with certain level of recombinant Visfatin (VF) and the recovery rates were calculated by comparing the measured value to the expected amount of Visfatin (VF) in samples.
　　Precision
　　Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Visfatin (VF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Visfatin (VF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
　　Linearity
　　The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Visfatin (VF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
　　Stability
　　The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
　　Reagents and materials provided
　　Assay procedure summary
　　1. Prepare all reagents, samples and standards; 2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC; 3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC; 4. Aspirate and wash 3 times; 5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC; 6. Aspirate and wash 5 times; 7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC; 8. Add 50μL Stop Solution. Read at 450nm immediay.
　　Test principle
　　The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Visfatin (VF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Visfatin (VF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Visfatin (VF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &plusmn; 10nm. The concentration of Visfatin (VF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.